Bio-Speedy® Avian Influenza Virus RT-qPCR Detection and Typing Kit is based on the detection of specific gene regions of Avian Influenza virus (AIV) subtypes by real-time reverse transcription polymerase chain reaction (RT-qPCR). For this purpose, the unknown nucleic acid isolate is first subjected to the “Influenza A Virus” targeted RT-qPCR. In the event of a positive result, in order to identify the detected Influenza A virus; the RT-qPCR process is repeated with oligonucleotides with specific labeling designed specifically for the genome of the target species. The specific Oligo Mixes to be used (Inf-H5-Oligo Mix with HEX stain, Inf-H7-Oligo Mix with Cy5 stain, A.Inf-N8-Oligo Mix with ROX stain, A.Inf-H9-Oligo Mix with FAM stain ) and Influenzae H5Nx, Influenzae H7Nx and Influenzae H9Nx virus hemagglutinin; Neurominidase gene regions of influenzae HxN8 viruses are targeted.
INTENDED USE
Bio-Speedy® Avian Influenza Virus RT-qPCR Detection and Typing Kit is used for molecular detection and identification of the causative agent of Avian Influenza (AI) in tissues, organs and swab samples from poultry.