The principle of the Bio-Speedy® Escherichia coli O157 Real-Time PCR Detection Kit is based on the detection of the rfbE (perosamine synthetase) gene of Escherichia coli O157 bacterial serotype by real-time polymerase chain reaction (qPCR). The kit also contains internal control reagents. With the internal control reactions (qPCR IC and qPCR IC-PC), sample-based inhibition control and kit reagent control are performed.
INTENDED USE
Bio-Speedy® Escherichia coli O157 Real-Time PCR Detection Kit is used for the determination of food and waterborne Escherichia coli O157 bacteria causing hemorrhagic colitis by molecular method.