Bio-Speedy® GAS Real-Time PCR Detection Kit is based on the detection of S. pyogenes pyrogenic exotoxin B (spEB) gene region with FAM-labeled oligonucleotides specific for S. pyogenes genome by Q-PCR in throat swab samples. Using heat-fragmentation, PCR inhibitor blocking peptides and kit components, quality DNA isolation that can be used in Q-PCR from swab within 10 minutes is possible. Oligonucleotides targeting the SPEB gene were used to specifically amplify Kitte S. pyogenes-specific DNA by Q-PCR. With Q-PCR, the detection time varies between 30-50 min depending on the characteristics of the instrument used.
INTENDED USE
Bio-Speedy® GAS Real-Time PCR Detection Kit is a qualitative in vitro medical diagnostic device used to detect S. pyogenes in throat swab samples to aid in the diagnosis of AGBHS pharyngitis cases. Detection is accomplished by total DNA isolation from throat swabs and Q-PCR amplification of a region specific to the S. pyogenes genome.