Bio-Speedy® Geneticaly Modified Organisms (GMO) p35S/tNOS/pFMV Real-Time PCR Screening Kit is based on the detection of sequences belonging to the 35S promoter (obtained from Cauliflower Mosaic Virus), NOS terminator (obtained from Agrobacterium tumefaciens bacteria) and FMV promoter (obtained from Figwort Mosaic Virus) by Real-time polymerase chain reaction (qPCR). As a result of the analysis with the kit, if any of the 35S, NOS and FMV targets are observed, the sample is determined to contain GMO and a “GMO positive” result is given. The kit also contains plant-targeted oligos and internal control reagents. Extraction control (whether plant DNA can be obtained) with the applied plant-targeted PCR (qPCR Plant); The internal control PCR (qPCR IC) also performs reagent control (test run) and sample-based inhibition control.
INTENDED USE
Bio-Speedy® Geneticaly Modified Organisms (GMO) p35S/tNOS/pFMV Real-Time PCR Screening Kit is used for the detection of genetically modified organism presence by detection of p35S, tNOS and pFMV elements in nucleic acid isolates obtained from samples.