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LogicSmart Zika Virus
01 Overview
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- Categories: Infectious Disease, MOLECULAR DIAGNOSTICS, PCR Test Portfolio, Sexually Transmitted Infection
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02 Details
Logix Smart™ Zika Test Kit
The Logix Smart™ Zika Virus (ZIKV) test uses our patented CoPrimer™technology to detect the presence or absence of ribonucleic acid (RNA) of Zika virus in a real-time RT-PCR kit that targets conserved regions in the Zika virus genome.
The test operates using a single step reverse transcription real-time PCR reaction in serum or plasma samples, collected alongside with urine, from patients suspected of Zika fever or Zika disease during acute stages of the disease that happen between 2 to 20 days after the onset of the symptomatic or non-symptomatic infection. The amplified product is then detected within 40 cycles by fluorescent dyes FAM (green) or Cal Fluor 610 (orange).
- Each Logix Smart Zika Virus Test kit contains a complete, ready-to-use master mix.
- A human RNaseP gene marker serves as an Internal Positive Control (IPC) to monitor the quality of each reaction and is designed to also detect inadequate samples.
- Nuclease Free Water is also provided for a negative control.
Intended Use
Zika virus testing is essential to aid the control and spread of virus prior to pregnancy, transfusion or transplantation, or sexual relations. The Logix Smart Zika Virus Test Kit is intended for molecular biology applications for diagnosis of Zika virus in human specimens such as serum, plasma, or urine. It is recommended for serum or plasma to be collected alongside with a urine sample.
This product is for export only and is not for sale in the United States.
The Logix Smart Zika Virus Test™ was designed and validated as a real-time RT-PCR kit that targets conserved regions in the Asian lineage of the Zika virus genome (PRVABC59), which seems to be the strain that has been most prevalent in the Americas.
This assay is a single step reverse transcription real-time reaction that can be broken down into 3 stages: sample preparation, reverse transcription, and the polymerase chain reaction with real-time monitoring. The assay has an internal positive control (IPC) that also acts as an extraction control to monitor the extraction, amplification, and detection steps.