Bio-Speedy® Respiratory Path Viruses Multiplex Real-Time PCR Screening Kit is based on the principle of amplification and detection by multiplex single step reverse transcription qPCR with labeled oligonucleotides designed for specific gene regions of interest of Influenza and Respiratory tract infections in the samples nasopharyngeal aspirate / lavage, throat swab specimens, bronchoalveolar lavage specimen, sputum specimens, etc. The INFOC-Oligo Mix contains the target oligos of Phocine Distemper Virus (PDV) is used for internal control purposes. The target gene sequence of the Phocine Distemper Virus, which is added to the sample during viral nucleic acid extraction from respiratory system samples, is presented with the “Bio-Speedy® Viral Nucleic Acid Isolation Kit” and the “RINA ™ M14 Viral Nucleic Acid Isolation Kit” developed for robotic systems. Nucleic acid extraction control, sample-based inhibition control and kit reagent control are performed by the internal control reaction.
INTENDED USE
Bio-Speedy® Respiratory Path Viruses Multiplex Real-Time PCR Screening Kit is a qualitative in-vitro medical diagnostic kit used to help identify infection cases which are Influenzae A 2009 H1, Influenzae A H3Nx, Influenzae A H5Nx, Influenzae A H7Nx, Human Bocavirus (HBoV), Enterovirus (EV), Human Parechovirus (HPeV), Adenovirus (AV), Human Coronavirus HKU, Human Coronavirus NL63, Human Coronavirus OC43, Human Coronavirus 229E, Human Metapneumovirus (HMPV), Respiratory Syncytial Virus A / B, Human Rinovirus, Parainfluenza 1, Parainfluenza 2, Parainfluenza 3, Parainfluenza 4. It is possible to obtain results in as little as 90 minutes by amplifying the gene fragments of the target viruses with single step reverse transcription qPCR and detecting it in real time.