Bio-Speedy® Salmonella Real- Time PCR Detection and Serotiyping Kit (Infantis/ Kentucky/ Enteritidis/ Senftenberg/ Hadar/ Typhimurium/ Mbandaka/ Gallinarium/ Pullorum) is based on the detection of specific gene regions of Salmonella serovars by real-time polymerase chain reaction (qPCR). For this purpose, “Salmonella spp” targeted qPCR is applied to an unknown bacterial isolate. Once the isolate is identified as Salmonella genus, qPCR analysis is repeated with other target-specific oligonucleotides (Oligo Mixes) for serovar detection. The implementation steps can be summarized as follows:
1. qPCR is applied to the bacterial isolate with “Salmonella spp- Oligo Mix”.
2. For serovar detection in positive isolates; QPCR is applied with appropriate Oligo Mixes to Infantis, Kentucky, Enteritidis, Senftenberg, Hadar / Mbandaka, Gallinarium / Pullorum, Typhimurium and Paratyphi-B.
3. If any of the qPCRs targeting the serovars “Infantis”, “Kentucky”, “Enteritidis”, “Senftenberg”, “Typhimurium” or “Paratyphi-B” are positive, the result is that the isolate is a positive serovar and the experiment is terminated.
4. If qPCR result is positive with “Hadar / Mbandaka-Oligo Mix”, “Hadar Oligo Mix” and “Mbandaka Oligo Mix” qPCR is applied to the isolate; serovar of isolate concluded as whichever qPCR result is positive.
5. If qPCR result is positive with “Gallinarium / Pullorum Oligo- Mix”, “NonSpecific Oligo Mix” qPCR is applied to the isolate; positive qPCR result indicates the isolate is biovar Gallinarium, negative result indicates the isolate is biovar Pullorum
INTENDED USE
Bio-Speedy® Salmonella Real- Time PCR Detection and Serotiyping Kit (Infantis/ Kentucky/ Enteritidis/ Senftenberg/ Hadar/ Typhimurium/ Mbandaka/ Gallinarium/ Pullorum) is used for the detection of Salmonella bacteria and differentiation of positive Salmonella spp. samples to detect Salmonella serovars (Infantis, Kentucky, Enteritidis, Senftenberg, Mbandaka, Hadar, Typhimurium, Gallinarum, Paratyphi_B) and biovars (Pullorum and Gallinarum).